DRAFT
September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
104
Notes
7. Confirm that the liquid is at the bottom of each well of the calibration plate. If not,
centrifuge the plate again at a greater rpm and for a longer time.
IMPORTANT! Do not allow the bottom of the calibration plates to become dirty.
Fluids and other contaminants that adhere to the bottom of the plates can
contaminate the sample block(s) and cause an abnormally high background signal.
8. Load calibration plate 1 into the instrument:
a. Open the instrument drawer.
b. Place calibration plate 1 in the sample block(s) so that the A1 position is at the
back-left corner.
c. Close the instrument drawer carefully.
Correct Incorrect
Liquid is at the
bottom of the well.
• Not centrifuged with enough force,
or
• Not centrifuged for enough time
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