Applied Biosystems StepOnePlus User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Glossary.fm
Glossary
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
138
amplification stage Part of the instrument run in which PCR produces amplification of the target. The
amplification stage is called a cycling stage in the thermal profile and consists of
denaturing, primer annealing, and polymerization steps that are repeated.
For quantitation experiments, fluorescence data collected during the amplification stage
are displayed in an amplification plot, and the data are used to calculate results. For
genotyping or presence/absence experiments, fluorescence data collected during the
amplification stage are displayed in an amplification plot, and the data can be used for
troubleshooting. See also cycling stage.
assay In the StepOne
™
and StepOnePlus
™
systems, a PCR reaction mix that contains primers
to amplify a target and a reagent to detect the amplified target.
Assay ID Identifier assigned by Applied Biosystems to TaqMan
®
Gene Expression Assays and
TaqMan
®
SNP Genotyping Assays.
assay information
file (AIF)
Data file on a CD shipped with each assay order. The file name includes the number from
the barcode on the plate. The information in the AIF is provided in a tab-delimited format.
assay mix PCR reaction component in Applied Biosystems TaqMan
®
Gene Expression Assays and
TaqMan
®
SNP Genotyping Assays. The assay mix contains primers designed to amplify
a target and a TaqMan
®
probe designed to detect amplification of the target.
AutoDelta In the run method, a setting to increase or decrease the temperature and/or time for a step
with each subsequent cycle in a cycling stage. When AutoDelta is enabled for a cycling
stage, the settings are indicated by an icon in the thermal profile:
•AutoDelta on:
•AutoDelta off:
automatic baseline An analysis setting in which the software calculates the baseline start and end values for
the amplification plot. You can apply the automatic baseline setting to specific wells in
the reaction plate. See also baseline.
automatic C
T
An analysis setting in which the software calculates the baseline start and end values and
the threshold in the amplification plot. The software uses the baseline and threshold to
calculate the threshold cycle (C
T
). See also threshold cycle (C
T
).
baseline In the amplification plot, a line fit to the fluorescence levels during the initial stages of
PCR, when there is little change in fluorescence signal.

Applied Biosystems StepOnePlus Specifications

General

Brand	Applied Biosystems
Model	StepOnePlus
Category	Laboratory Equipment
Language	English