DRAFT
September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Infrequent Maintenance
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
116
Notes
2. Using the data from the background calibration, identify the location of the
contaminated wells on the sample block(s).
3. Turn off and unplug the system. Allow it to cool for 15 min.
4. Open the instrument drawer.
5. Clean each contaminated well of the sample block(s):
a. Pipette a small volume of water into the contaminated well, slowly pipette the
water up and down several times, then expel the water into a waste beaker.
b. Use a cotton swab to scrub inside of the contaminated well.
c. Use a lint-free cloth to absorb the excess deionized water in the well.
d. Repeat steps 5a through 5d.
6. Close the instrument drawer, then plug in and power on the instrument.
7. Perform a background calibration to confirm that you have eliminated the
contamination.
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