Applied Biosystems StepOnePlus User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Glossary.fm
Glossary
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
142
IPC+ See negative control-IPC wells.
made-to-order
assays
TaqMan
®
Gene Expression Assays or TaqMan
®
SNP Genotyping Assays that are
manufactured at the time of order. Only assays that pass manufacturing quality control
specifications are shipped.
manual baseline An analysis setting in which you enter the baseline start and end values for the
amplification plot. You can apply the manual baseline setting to specific wells in the
reaction plate.
manual C
T
An analysis setting in which you enter the threshold value and select whether to use
automatic baseline or manual baseline values. The software uses the baseline and the
threshold values to calculate the threshold cycle (C
T
).
melt curve A plot of data collected during the melt curve stage. Peaks in the melt curve can indicate
the melting temperature (Tm) of the target or can identify nonspecific PCR amplification.
In the StepOne
™
software, you can view the melt curve as normalized reporter (Rn) vs.
temperature or as derivative reporter (−Rn′ ) vs. temperature. Also called dissociation
curve.
melt curve stage In the thermal profile, a stage with a temperature increment to generate a melt curve.
melting
temperature (Tm)
In melt curve experiments, the temperature at which 50% of the DNA is double-stranded
and 50% of the DNA is dissociated into single-stranded DNA. The Tm is displayed in the
melt curve.
multicomponent
plot
A plot of the complete spectral contribution of each dye for the selected well(s) over the
duration of the PCR run.
negative control
(NC)
In the StepOne
™
software, the task for targets or SNP assays in wells that contain water
or buffer instead of sample. No amplification of the target should occur in negative control
wells. Previously called no template control (NTC).
negative control-
blocked IPC wells
In presence/absence experiments, wells that contain IPC blocking agent instead of sample
in the PCR reaction. No amplification should occur in negative control-blocked IPC wells
because the reaction contains no sample and amplification of the IPC is blocked.
Previously called no amplification control (NAC).
negative control-
IPC wells
In presence/absence experiments, wells that contain IPC template and buffer or water
instead of sample. Only the IPC template should amplify in negative control-IPC wells
because the reaction contains no sample. Previously called IPC+.
no amplification
control (NAC)
See negative control-blocked IPC wells.
no template control
(NTC)
See negative control (NC).

Applied Biosystems StepOnePlus Specifications

General

Brand	Applied Biosystems
Model	StepOnePlus
Category	Laboratory Equipment
Language	English