Getting Started Page 23 of 42
Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.
Incubate a Plate
From the Main Menu, tap Quick and select the Incubate tab.
1. Check the Active box.
2. Define a Set point from 18°C to 65°C (64.4°F to 149°F). Heating begins immediately.
3. Move the plate carrier in, if it is currently out.
4. When the temperature displayed at the top of the screen reaches the set point, move
the carrier out, insert the plate, and move the carrier back in.
The minimum set point must be ambient room temperature plus 4°C.
Condensation Control™
A temperature Gradient can be applied to reduce the risk of condensation when using
microplate lids. When no gradient (0) is applied, the heaters above and below the
plate operate at the same (setpoint) temperature. This is recommended if the plate
has no lid, or condensation is not a problem when lids are used.
When a gradient (0.1 to 2.0) is applied, the heaters above the plate operate slightly
higher than the set point and the heaters below the plate operate slightly lower than
the set point. The gradient represents the temperature separation between the top
and bottom heaters. This differential should serve to reduce or eliminate
condensation.
In the example below, the Set point is 37°C and the Gradient is 1.0:
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