Getting Started Page 27 of 42
Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.
Spectral Scan Protocols
Plate: Options include standard 6-, 12-, 24-, 48-, 96-, and 384-well plates, Cuvette (if
equipped), and Take3 (if configured).
Start WL and End WL: Define the start and end wavelengths. The full spectral range is
200 to 999 nm.
Step Size: Define an increment (in nm) for measurement. For example, if the Start WL
is 200 and the Step Size is 10, measurements are taken at 200, 210, 220, and so on
until the End WL is reached.
The number of spectral data points cannot exceed 100.
Microplates and Take3 only:
Calibrate first: Set to Yes (optimum performance) to perform calibration at all of the
specified wavelengths when a plate read is initiated. Set to No (optimum speed) to
perform calibration at only those wavelengths not yet calibrated since the reader was
turned on.
Shake Before Read: Select a shake mode of Linear (side-to-side), Orbital (circle), or
Double Orbital (figure 8). Set a Duration (up to 2 hours 45 minutes) to shake the plate
before spectral scanning begins. Adjust the Frequency, if needed. The cycles per
minute updates as the slider moves. The measurement in mm indicates the distance
the carrier travels during the shake.
When finished, tap Save. The protocol name is added to the Main Menu.
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