Specifications Page 41 of 42
Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.
Linearity
By liquid dilution, 200 µL volume in 96-well plate, 80 µL volume in 384-well plate
96-well plate
normal read speed
delay after plate movement = 100 ms
0.000 to 2.000 OD: ±1.0% ±0.010 OD
2.000 to 2.500 OD: ±3.0% ±0.010 OD
384-well plate
normal read speed
delay after plate movement = 100 ms
0.000 to 1.500 OD: ±2.0% ±0.010 OD
1.500 to 2.000 OD: ±5.0% ±0.010 OD
96- and 384-well plate
sweep read speed
0.000 to 1.000 OD: ±1.0% ±0.010 OD
Repeatability (Standard Deviation [STD])
Tested with certified neutral density glass, measured by one standard deviation
(8 measurements/data point)
96-well plate
normal read speed
delay after plate movement = 100 ms
0.000 to 2.000 OD: ±1.0% ±0.005 OD
2.000 to 2.500 OD: ±3.0% ±0.005 OD
384-well plate
normal read speed
delay after plate movement = 100 ms
0.000 to 1.500 OD: ±1.0% ±0.005 OD
1.500 to 2.000 OD: ±3.0% ±0.005 OD
96- and 384-well plate
sweep read speed
0.000 to 1.000 OD: ±2.0% ±0.010 OD
Assay Validation
Using a Take3 plate
260 nm dsDNA detection limit: < 5 ng/μL
Cuvette
Absorbance performance of measurements made via cuvette shall satisfy the
requirements given above for accuracy, linearity. and repeatability in a 96 well-plate,
normal read speed, 100 ms delay after movement.
To Next Page
Loading...
