132 | Chapter 6: Instrument Qualification
Measurements per Data Point: 50
Dynamic Range: Standard
Light Source: Tungsten
Dispense Module Tests
Applies only to Synergy HTX models with injectors.
BioTek has developed a set of tests that you can perform to ensure that the dispense
module performs to specification initially and over time. We recommend that you perform
these tests before first use (e.g., during the Initial OQ), and then every three months.
• The
Accuracy Test is a measure of the mean volume per well for multiple
dispenses. The actual weight of the dispensed fluid is compared to the expected
weight and must be within a certain percentage to pass. Pass/Fail criteria depends
on the per-well volume dispensed: 2.0% for 80 µL, 5.0% for 20 µL, and 20.0% for
5 µL. It is assumed that one gram is equal to one milliliter.
The test uses a single green dye test solution and one 96-well microplate (per
injector) to test the three different volumes. The balance is tared with the empty
plate, and then the 80 µL dispense is performed for columns 1–4. The fluid is
weighed and the balance is tared again (with the plate on the balance). This process
is repeated for the 20 µL and 5 µL dispenses. It is assumed that the solutions used
are at room temperature. A precision balance (three-place) is used to weigh the
plate.
• The
Precision Test is a measure of the variation among volumes dispensed to
multiple wells. For each volume dispensed (80 µL, 20 µL, and 5 µL) to four
columns, the %CV (coefficient of variation) of 32 absorbance readings is calculated.
Pass/Fail criteria depends on the per-well volume dispensed: 2.0% for 80 µL, 7.0%
for 20 µL, and 10.0% for 5 µL. The plate is read in an absorbance reader at 405/750
nm for columns 1–4 and at 630/750 nm for columns 5–12.
The two tests are performed simultaneously and use the same plate.
Failures
If any tests fail, prime the fluid lines and rerun the test(s).
If the test(s) fail again, the injector may require cleaning. See
Preventive Maintenance.
If tests continue to fail, contact BioTek’s Technical Assistance Center.
BioTek Instruments, Inc.
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