Biotek Synergy HTX User Manual

122 | Chapter 6: Instrument Qualification
• Aspirate 150 µL from wells C4-F4 and dispense into wells C5-F5.
Mix the wells using the pipette.
• Aspirate 150 µL from wells C5-F5 and discard.
1 2 3 4 5 6 7 8 9 10 11 12
A
3.3 nM 3.3 nM 3.3 nM
CBUF CBUF
3.3 nM 3.3 nM 3.3 nM
B
CBUF* CBUF CBUF CBUF CBUF CBUF CBUF CBUF
C
150µL:
1.0 nM
0.5 nM
0.25
nM
0.125
nM
0.0625
nM
BUF
D 1.0 nM 0.5 nM
0.25
nM
0.125
nM
0.0625
nM
200µL:
1.0 nM
BUF
E 1.0 nM 0.5 nM
0.25
nM
0.125
nM
0.0625
nM
BUF
F 1.0 nM 0.5 nM
0.25
nM
0.125
nM
0.0625
nM
G
CBUF CBUF CBUF CBUF CBUF CBUF CBUF CBUF
H
3.3 nM 3.3 nM 3.3 nM
CBUF CBUF
3.3 nM 3.3 nM 3.3 nM
CBUF applies only to the Hellma Quartz plate.
Gen5 Protocol Reading Parameters
The following tables contain the recommended settings for the Gen5 protocols. Your
tests may require modifications to some of these parameters, such as Plate Type or
Gain (see “Troubleshooting” on page 120).
 Adjust the Plate Type setting to match the plate you are actually
using, if different from shown here.
BioTek Instruments, Inc.

Biotek Synergy HTX Specifications

General

Brand	Biotek
Model	Synergy HTX
Category	Laboratory Equipment
Language	English