Fluorescence Specifications | 147
Fluorescence Specifications
Read Timing
Because of the possible wide variations in setup, the following benchmark
conditions are specified:
Excitation Filter: 485/20 nm
Emission Filter: 528/20 nm
Measurements per data
point:
10
Delay after plate
movement:
100 ms
Minimum kinetic interval,
96-well
<55 seconds
Optical Probes
The Synergy HTX is configured with two probe sizes: The 3 mm probes can be
installed only in the top position, and the 5 mm probe can be installed only in the
bottom position.
Sensitivity
Specifications apply to the Normal mode of reading
5 mm optical probe
Bottom reading
DL Sodium Fluorescein in PBS
<= 30 pM
Excitation 485/20, Emission 528/20
DL Propidium Iodide in PBS
<= 62.5 ng/mL
Excitation 485/20, Emission 645/40
3 mm optical probe
Top reading
DL Methylumbelliferone in CBB
<=0.16 ng/mL
Excitation 360/40, Emission 460/40
Optional Time-Resolved Fluorescence (“T” models)
Delay: 0, or 20 to 2,000 µs
Integration Time: 20 to 2,000 µs
Granularity: 10-µs step
Synergy HTX Operator’s Manual
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