Gen5 Software | 41
Priming the System
Before an assay requiring fluid dispense is run, the system should be fully primed
with the reagent or other fluid used by the assay. At the start of the assay (and
optionally at the start of each dispense to a well), an additional injector tip prime
can be performed. The tip prime compensates for any fluid loss at the injector tip
due to evaporation since the last dispense. All priming activities are controlled via
Gen5 (see page 46).
Both types of primes require a fluid reservoir to be present on the microplate
carrier:
• The priming plate is about the same size as a standard microplate and is
placed on the microplate carrier for a Prime operation (to prime the
dispense system with fluid).
• The tip priming trough is a small, removable priming cup located in the left
rear of the carrier, and is used for performing the Tip Prime before
dispensing. The trough holds up to 1.5 mL of liquid and must be
periodically emptied and cleaned by the user.
Figure 18: Priming plate and tip priming trough
Gen5 Software
BioTek’s Gen5 software supports all Synergy HTX reader models. Use Gen5 to control the
reader and the dispense module, perform data reduction and analysis on the measurement
tip priming
trough
Synergy HTX Operator's Manual
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