2 | Chapter 1: Introduction
Synergy HTX Multi-Mode Microplate Reader
The Synergy HTX is a single-channel microplate reader available with absorbance,
fluorescence, and luminescence detection. It is computer-controlled using BioTek’s Gen5
software for all operations including data reduction and analysis. Synergy HTX is robot
accessible and compatible with BioTek’s BioStack Microplate Stacker.
When making fluorescence determinations, the Synergy HTX uses a tungsten quartz
halogen lamp with interference filters for wavelength specificity in conjunction with a
photomultiplier (PMT) tube detector. The Synergy HTX has both top and bottom probes
for fluorescence measurements. The top probe can be adjusted vertically for the correct
reading height, via Gen5’s Read Height reading parameter (see
Chapter 3, Getting
Started
).
Luminescence is measured by the low-noise PMT detector through an empty filter position
in the Emission filter wheel. A filter can also be left in place if light filtering is necessary.
Absorbance measurements are made by switching to a xenon flash lamp and a
monochromator for wavelength selection. The use of a xenon flash lamp allows for both
UV and visible light absorbance measurements. The monochromator provides wavelength
selection from 200 to 999 nm in 1-nm increments.
The Synergy HTX has a 4-Zone temperature control from 4°C over ambient to 50°C,
controlled via a software-adjustable gradient. Internal plate shaking, with both linear and
orbital modes, is supported to ensure that reagents are properly mixed prior to reading.
Both Synergy HTX models support the reading of 6-, 12-, 24-, 48-, 96-, and 384-well
microplates with standard 128 x 86 mm geometry, as well as the BioTek Take3 and Take3
Trio Micro-Volume Plates. Absorbance mode reads plates up to 0.8" (20.3 mm) in height;
fluorescence mode reads plates up to 1.25" (31.75 mm). Polymerase Chain Reaction (PCR)
tubes up to 1.25" (31.75 mm) are also readable with the use of existing adapter plates.
For models with time-resolved fluorescence (TRF) capability, the TRF option allows
measurements by using the xenon flash light source in conjunction with the PMT
measurement detector. A special cartridge installed in the Excitation filter wheel location is
required.
Models with injectors support dual-reagent dispensing to 6-, 12-, 24-, 48-, 96-, and 384-well
microplates with standard 128 x 86 mm geometry. An external dispense module pumps
fluid from the supply bottles to the two injectors located inside the instrument. Both
injectors are positioned directly above the bottom probe, and fluid is injected into one well
at a time.
BioTek Instruments, Inc.
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