Absorbance Specifications | 145
Absorbance Specifications
Accuracy, Linearity, Repeatability
All qualifications were conducted using 96-/384-well, flat-bottom microplates.
For the performance described here, the Gain on the Optics Test should be
below 10.0.
Measurement Range: 0.000 to 4.000 OD
Accuracy (tested with certified neutral density glass):
96-well plate, normal read speed:
0.000 to 2.000 OD ±1.0% ±0.010 OD, Delay after plate movement: 100 ms
2.000 to 3.000 OD ±3.0% ±0.010 OD, Delay after plate movement: 100 ms
384-well plate, normal read speed:
0.000 to 2.000 OD ±2.0% ±0.010 OD, Delay after plate movement: 100 ms
2.000 to 2.500 OD ±3.0% ±0.010 OD, Delay after plate movement: 100 ms
96-well and 384-well plate, sweep read speed:
0.000 to 1.000 OD ±1.0% ±0.010 OD
Linearity (by liquid dilution):
96-well plate, normal read speed:
0.000 to 2.000 OD ±1.0% ±0.010 OD, Delay after plate movement: 100 ms
2.000 to 3.000 OD ±3.0% ±0.010 OD, Delay after plate movement: 100 ms
384-well plate, normal read speed:
0.000 to 2.000 OD ±2.0% ±0.010 OD, Delay after plate movement: 100 ms
2.000 to 2.500 OD ±3.0% ±0.010 OD, Delay after plate movement: 100 ms
96-well and 384-well plate, sweep read speed:
0.000 to 1.000 OD ±1.0% ±0.010 OD
Repeatability (tested with certified neutral density glass measured by
one standard deviation (8 measurements per data point):
96-well and 384-well plate, normal read speed:
0.000 to 2.000 OD ±1.0% ±0.005 OD, Delay after plate movement: 100 ms
2.000 to 3.000 OD ±3.0% ±0.005 OD, Delay after plate movement: 100 ms
96-well and 384-well plate, sweep read speed:
0.000 to 1.000 OD ±2.0% ±0.010 OD
Synergy HTX Operator’s Manual
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