Chapter 4 Maintenance
4.1 Self Diagnosis and Automatic Adjustment
The HALO DB-20 spectrophotometer will execute self-diagnosis and
automatic adjustment immediately after the power switch is turned on. For
details, refer to chapter 3.
4.2 Confirmation of Specifications
4.2.1 Wavelength Accuracy
1. Verify that the instrument is powered up and wait for 30 minutes at least.
2. Select Wavelength Scan from the Main Menu.
3. Set the operating conditions as shown below:
Data Mode E(R)
Start WL (nm) 660
Stop WL (nm) 650
Scan Speed (nm/min) 100
UP Scale 100
LO Scale 0
Lamp Change WL (nm) 340.0
WI Lamp ON
D2 Lamp ON
Response Fast
4. Verify that the Sample and Reference cuvette holders are empty. Then
press 0 to display the measurement screen.
5. Press START to acquire a spectrum.
6. After the measurement, read the peak wavelength.
7. If the wavelength position is within the range of 656.1
±0.3nm, it is
normal. If the peak is not in the range then:
a) Change the existing scale to provide an easier to read graph and if
the peak is still not within range, perform the WL Calibration in
Chapter 3.
b) Once you have performed the wavelength calibration, repeat steps
necessary to confirm that the peak is within the specified normal
value range.
4-1
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