Dynamica HALO DB-20 - Special Samples; Functions; Quantitative Analysis

2-4
Low volatility.
Table 2-1 lists the applicable wavelength ranges of common organic solvents.
Table 2-1 Wavelength Ranges for Common Solvents
Solvent Wavelength Range
Cyclohexane 200nm ~ 400
+
nm
Ethyl alcohol 220 nm ~ 400
+
nm
Methyl alcohol 220 nm ~ 400
+
nm
Ethyl ether 220 nm ~ 400
+
nm
Dioxane 220 nm ~ 400
+
nm
Hexane 220 nm ~ 400
+
nm
Chloroform 250 nm ~ 400
+
nm
Isopropyl alcohol 250 nm ~ 400
+
nm
Acetic acid 250 nm ~ 400
+
nm
Ethyl acetate 270 nm ~ 400
+
nm
Carbon tetrachloride 275 nm ~ 400
+
nm
Benzene 280 nm ~ 400
+
nm
Methyl ethyl ketone 335 nm ~ 400
+
nm
Acetone 340 nm ~ 400
+
nm
Carbon disulfide 380 nm ~ 400
+
nm
2.3.2 Special Samples
Note that the laws expressed by Equations (1) and (2) are not applicable to the
following special samples.
Fluorescing sample
Appreciably turbid sample
In measurement of a solid sample as glass plate, the energy of beam radiation
undergoes a loss (r) due to reflection on the surface of solid substance.
In this case, Equation (1) must be transformed as shown below.
I
t
/I
0
= 10
-ε.c.l
– r ………………………………………………. …(3)
Where, ‘r’ varies depending on the reflectance of substance.
2.4 Functions
2.4.1 Quantitative Analysis