5.1 Before you prepare the system
Introduction
It is important to prepare the system in accordance with the settings in the method to
be run. Before preparing the system, check the settings in the Method Editor and make
sure that all accessories to be used are available.
Checklist
Make sure the system is prepared in accordance with the settings in the method to be
run. Depending on configuration, remember to check:
•
which valve ports to use for inlets and outlets
•
which column type to use
•
which column position to use
•
which buffers and samples to prepare
•
which sample application technique to use
•
that the pH electrode is connected and calibrated
CAUTION
Reversed Phase Chromatography (RPC) runs with 100% acetoni-
trile in ÄKTA pure. Always replace the PEEK tubing between the
used system pump and the pump pressure monitor with orange
PEEK tubing, i.d. 0.5 mm, before running RPC with 100% acetonitrile.
The tubing to be replaced is green for 25 ml/min systems and beige
for 150 ml/min systems. Set the System pressure alarm to 10 MPa
for 25 ml/min systems.
ÄKTA pure Operating Instructions 29022997 AF 103
5 Prepare the system for a run
5.1 Before you prepare the system
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