GE akta pure - 6.2 Applying the Sample

6.2 Applying the sample
Introduction
A number of different sample application techniques are available. This section describes
sample application using a syringe to manually fill a s loop. The two stages of the sample
application are described in the table below. For detailed instructions and information
regarding the different sample applications techniques, see ÄKTA pure User Manual.
DescriptionStage
The sample loop is filled with sample.Load
The sample is injected onto the column.Inject
How to fill a sample loop
Follow the instructions below to fill the sample loop with sample.
ActionStep
Connect a suitable sample loop to the injection valve ports LoopF (fill) and
LoopE (empty).
1
Fill a syringe with sample.2
122 ÄKTA pure Operating Instructions 29022997 AF
6 Run a method
6.2 Applying the sample