ActionStep
Connect the syringe to the injection valve port Syr.3
Open the System Control module.4
In the Process Picture:5
•
Click on the Injection valve and select Manual load.
Result: The injection valve switches to manual load position.
Load sample into the sample loop. To avoid sample loss due to siphoning,
leave the syringe in the port until the sample has been injected onto the
column during the run.
Tip:
It is recommended to overload the loop to make sure that the loop is com-
pletely filled. Excess of sample will leave the valve through port W1.
6
ÄKTA pure Operating Instructions 29022997 AF 123
6 Run a method
6.2 Applying the sample
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