4.5 Prime inlets and purge pump heads
Introduction
Before usage of the system pumps, it is important to:
•
Prime the inlets (fill the buffer inlets with liquid).
•
Purge the system pumps (remove air from the pump heads).
Note that the procedures described in this section may have to be adapted if
your system configuration differs from the one described in this manual.
Note:
Overview
The procedure consists of the following stages:
DescriptionStage
Prime all inlet tubing to be used during the run1
Purge System pump B2
Validate purge of System pump B3
Purge System pump A4
Validate purge of System pump A5
End the run6
The procedures for purging the pump heads and priming the inlets using the
Process Picture, are described below. It is also possible to perform the proce-
dures from the Manual instructions dialog.
Tip:
Prime inlet tubing
Follow the instructions below to fill all A and B inlet tubing to be used in the run with
appropriate buffer/solution.
ActionStep
Make sure that all inlet tubing that is to be used during the method run is
placed in the correct buffer.
1
Open the System Control module.2
ÄKTA pure Operating Instructions 29022997 AF 91
4 Installation
4.5 Prime inlets and purge pump heads
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