6.1 Before you start
Introduction
Before starting a run, it is necessary to read and understand the information in this
section and to perform the checks listed below.
CAUTION
Reversed Phase Chromatography (RPC) runs with 100% acetoni-
trile in ÄKTA pure. Always replace the PEEK tubing between the
used system pump and the pump pressure monitor with orange
PEEK tubing, i.d. 0.5 mm, before running RPC with 100% acetonitrile.
The tubing to be replaced is green for 25 ml/min systems and beige
for 150 ml/min systems. Set the System pressure alarm to 10 MPa
for 25 ml/min systems.
CAUTION
Always use appropriate personal protective equipment during op-
eration and maintenance of ÄKTA pure.
CAUTION
Hazardous substances. When using hazardous chemical and bio-
logical agents, take all suitable protective measures, such as
wearing protective glasses and gloves resistant to the substances
used. Follow local and/or national regulations for safe operation,
maintenance and decommissioning of the equipment.
CAUTION
High pressure. ÄKTA pure operates under high pressure. Wear
protective glasses at all times.
CAUTION
Risk of explosion. Do not use Mixer chamber 15 ml in the low flow
system. The maximum pressure for Mixer chamber 15 ml is 5 MPa.
ÄKTA pure Operating Instructions 29022997 AF 119
6 Run a method
6.1 Before you start
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