Checklist
Make sure that the system is correctly prepared. Check that:
•
The system is prepared according to the settings in the method to be run.
•
A suitable column has been selected for the application (consider target protein and
pressure range).
•
The buffer inlet tubing is immersed in correct buffer vessels (consider solution iden-
tity and volume).
•
All waste tubing is immersed in appropriate waste vessels (consider vessel size,
placement and material).
•
No tubing is twisted and the flow path is free from leakage.
Hold, pause or stop the run
At the end of a method the run stops automatically. All pumps stop and an acoustic end
signal sounds and End is displayed in the Run Log.
To interrupt a method during a run you may use the Hold, Pause or End icons in System
Control. A held or paused method run can be resumed by using the Continue icon. See
the instructions in the table below.
then...If you want to...
click the Hold icon.temporarily hold the method, with current
flow rate and valve positions sustained
click the Pause icon.temporarily pause the method, and stop
all pumps
click the Continue icon.resume, for example, a held or paused
method run.
Note:
An ended method cannot be continued.
120 ÄKTA pure Operating Instructions 29022997 AF
6 Run a method
6.1 Before you start
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