Sample application through a
sample loop
The method for how to apply a sample can be created beforehand, see Section 6.3 Start
a method run, on page125. During sample application, the sample is automaticallyinjected
onto the column and the loop is then emptied and washed out using buffer from the
system pumps. The total buffer volume to be used for emptying and washing the sample
loop is set in the Method Editor, in the Phase Properties tab of the Sample Application
phase at Empty loop with.
Empty the sample loop with a buffer volume that exceeds the volume of the
loop. This will ensure that the loop is completely emptied.
Tip:
124 ÄKTA pure Operating Instructions 29022997 AF
6 Run a method
6.2 Applying the sample
To Next Page
Loading...
