Attach a column holder and
connect a column
Follow the instructions below to connect a column to the instrument. Always use a column
holder. If a column valve is used, connect the column to the appropriate A and B ports
on the valve. If no column valve is used, connect the column directly to the flow path
tubing. Use appropriate tubing and connectors. The instructions below show a system
configured with Column valve V9-Cs.
ActionStep
Attach an appropriate column holder to the rail on the instrument.1
Attach the column to the column holder.2
Connect a suitable tubing to a Column valve port, in this example port 1A.3
Open the System Control module.4
110 ÄKTA pure Operating Instructions 29022997 AF
5 Prepare the system for a run
5.4 Connect a column