ActionStep
•
Select Tubing and Delay Volumes
and
•
select Tubing: Injection valve to column
2
•
Select the inner diameter of the tubing between the injection valve and
the column from the I.D. drop-down list.
•
Type in the tubing Length.
3
If the sample pump is used:4
•
Select Tubing: Sample pump to injection.
•
Set tubing i.d. and length, see step 3.
Click OK.
Note:
The system now calculates the Pre-column pressure.
5
Pre-column pressure alarms
It is important that the pre-column pressure alarm is set during all runs where a column
is used. The pressure alarm can be set in:
•
the method to be run,
•
the System Settings dialog , or
•
during a manual run
Pre-column pressure alarm limits are automatically set in the method when a column
from the column list is selected in the method. Refer to UNICORN Method Manual for
more information on pressure alarms.
ÄKTA pure Operating Instructions 29022997 AF 115
5 Prepare the system for a run
5.5 Pressure alarms
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