Images of pH probe
The left image below shows the pH probe located for storage and calibration. A cup has
been placed in the cup holder.
The right image below shows the pH flow cell for operation.
Off-line pH calibration
The table below describes how to calibrate the pH probe.
Always have one end of the grounding strip submerged in the buffer during
calibration.
Note:
ActionStep
Prepare two cups with buffers representing the actual required pH range.1
Place the cup with the highest pH solution in the cup holder. Insert the pH
probe into the holder, with the end submerged in the buffer.
Note:
Attach the cap to the pH flow cell to prevent foreign objects from entering
the flow path.
2
Select System:Calibrate in UNICORN System Control module.3
Enter the pH value for the first buffer (with the highest pH solution) in the
Reference value 1 field, wait for the value to stabilize, and press Read value
1.
4
Remove the pH probe from the holder and rinse it with distilled water.5
ÄKTAprocess Operating Instructions 29-0252-49 AA 149
6 Maintenance
6.5 Calibration of pH probe
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