5.1.2 Prepare system components
Introduction
This section outlines the steps needed to prepare the pH meter, UV monitor and air
sensors before a run.
pH calibration
The pH meter is removed from the flow path for calibration. Calibrate the pH meter ac-
cording to Section 6.5 Calibration of pH probe, on page 148.
Install the pH electrode
Follow the instruction below to install the pH electrode into the pH flow cell.
CAUTION
Make sure that the pH electrode is mounted correctly after reassem-
bly.
Organic solvents will cause pH electrode degeneration. When running methods
using organic solvents it is recommended that the pH electrode is not inserted
in the flow cell.
Note:
ActionStep
Unscrew the plastic fastening nut from the pH cell and remove the pH plug.1
Move the O-ring from the pH plug to the electrode adapter.2
Remove the pH electrode adapter from its holder.3
If the electrode is not mounted in the adapter, remove the protective cap,
rinse the electrode with pure water and insert the electrode into the adapter.
Note:
The pH plug may be placed in the pH electrode holder while the electrode is
in use.
4
ÄKTAprocess Operating Instructions 29-0252-49 AA 115
5 Operation
5.1 Prepare the system
5.1.2 Prepare system components
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