Hettich AG, Seestrasse 204a, 8806 Baech, Switzerland Operator_Manual_Rotolavit II_und_Rotolavit II-S_en_rev2.5
Seite 32
9.3.3 wash redcells 3 5ml 3x (wash erythrocytes, 3.5 ml, 3 x)
This preinstalled program is visualized as an example.
The process values are:
FILL 3.5ml 1100rpm (fill with 3.5 ml by 1100rpm)
SPIN 20sec 3500rpm (acceleration 800rpm/s), braking 1000rpm/s)
DECANT 390rpm (decant by 390rpm)
AGIT 15x (15 agitations)
LOOP 3x (two more runs, a total of three wash cycles)
9.3.4 agit and spin
The process values are:
AGIT 15x (15 agitations)
SPIN 20sec 3500rpm (acceleration 800rpm/s, braking 1000rpm/s)
9.3.5 decant
The process values are:
DECANT 390 rpm (decant by 390rpm)
9.3.6 spin 20sec 3500rpm
The process values are:
SPIN 20sec 3500rpm (acceleration 800rpm/s, braking 1000rpm/s)
9.3.7 susp 3 5ml spin 20sec (centrifuge 3.5ml suspension for 20sec)
The process values are:
FILL 3.5ml 1100rpm (fill 3.5 ml at 1100rpm)
SPIN 20sec 3500rpm (acceleration 800rpm/s, braking 1000rpm/s)
LOOP 2 of 3
(two more runs)
LOOP 3 of 3
(one more run)