Hettich Rotolavit II User Manual

Hettich AG, Seestrasse 204a, 8806 Baech, Switzerland Operator_Manual_Rotolavit II_und_Rotolavit II-S_en_rev2.5
Seite 33
9.3.8 wash 3 5ml 3x and anti (wash 3.5 ml, 3 x, plus antihuman globulin test)
The process values are:
 FILL 3.5ml 1100rpm (fill 3.5ml by 1100rpm)
 SPIN 20sec 3500rpm (acceleration 800rpm/s, braking 1000rpm/s)
 DECANT 390rpm (decant by 390rpm)
 AGIT 15x (15 agitations)
 LOOP 3x (two more runs)
 CHECK Pause (to add the antihuman globulin manually)

 SPIN 20sec 3500rpm (acceleration 800rpm/s, braking 1000rpm/s)
9.3.9 wash white cells Tspot (wash leukocytes, Tspot)
The process values are:
 FILL 2.5ml 900rpm (fill 2.5ml by 900rpm)
 SPIN 7min 2260rpm (acceleration 800rpm/s, braking 1000rpm/s)
 DECANT 370rpm (decant by 370rpm)
 AGIT 100x (100 agitations)
 LOOP 2x (only one more run)
9.3.10 cell recovery (only device type 1008-00S)
The process values are:
 FILL 2.0ml 1100rpm (fill 2.0ml by 1100rpm)
 SPIN 4min 2260rpm (acceleration 800rpm/s, braking 1000rpm/s)
 DECANT 370rpm (decant by 370rpm)
 AGIT 50x (50 agitations)
 LOOP 2x (only one more run)
 FILL 2.6ml 1100rpm (fill 2.6ml by 1100rpm)
9.2.11 immunophenotyping (only device type 1008-00S)
The process values are:
 FILL 1.5ml 1100rpm (fill 1.5ml by 1100rpm)
 SPIN 5min 1850rpm (acceleration 800rpm/s, braking 1000rpm/s)
 DOWN 1100rpm
 DECANT 370rpm (decant by 370rpm)
 AGIT 15x (15 agitations)
 LOOP 2x (only one more run)
 FILL 0.5ml 1100rpm (fill 0,5ml by 1100rpm)

Hettich Rotolavit II Specifications

General

Brand	Hettich
Model	Rotolavit II
Category	Laboratory Equipment
Language	English