Alternatively, select Use the following IP address to connect the instrument to another server manually as
follows. Contact your network administrator for the addresses specific to your facility.
u Enter IP address. The IP address is a series of 4 numbers separated by a dot, similar to
168.62.20.37, for example.
u Enter the subnet mask, which is a subdivision of the IP network.
u Enter the default gateway, which is the router on the network that connects to the internet.
4 Select Obtain a DNS server address automatically to connect the instrument to the domain name server
associated with IP address.
Alternatively, select Use the following DNS server addresses to connect the instrument to the domain
name server manually as follows.
u Enter the preferred DNS address. The DNS address is the server name used to translate domain
names into IP addresses.
u Enter the alternate DNSaddress. The alternate is used if the preferred DNS cannot translate a
particular domain name to an IP address.
5 Select Save to advance to the Computer screen.
NOTE
The instrument computer name is assigned to the instrument computer at the time of manufacture. Any
changes to the computer name can affect connectivity and require a network administrator.
6 Connect the instrument computer to a domain or a workgroup as follows.
u For instruments connected to the internet—Select Member of domain, and then enter domain name
associated with the internet connection at your facility. Domain changes require an administrator user
name and password.
u For instruments not connected to the internet—Select Member of work group, and then enter a work
group name. The work group name is unique to your facility.
7 Select Save.
Set BaseSpace Configuration
1 From the Manage Instrument screen, select System Configuration.
2 Select BaseSpace Configuration.
3 Select from the following options to specify a location where data are transferred for subsequent analysis.
u Select BaseSpace to send sequencing data to Illumina BaseSpace. [Optional] Select the Output
Folder checkbox, select Browse, and navigate to a secondary network location to save BCL files in
addition to BaseSpace.
u Select BaseSpace Onsite. In the Server Name field, enter the full path to your BaseSpace Onsite
server. [Optional] Select the Output Folder checkbox, select Browse, and navigate to a secondary
network location to save BCL files in addition to the BaseSpace Onsite server.
u Select Standalone instrument to save data to a network location only. Select Browse and navigate to
a preferred network location. The control software generates the output folder name automatically.
u [Optional] Select Use Run Monitoring to monitor the run using visualization tools on BaseSpace. A
BaseSpace login and internet connection is required.
4 Select Save to advance to the BaseSpace screen.
5 If you selected BaseSpace or BaseSpace Onsite, set the BaseSpace parameters as follows.
u Enter a BaseSpace User Name and Password to register the instrument with BaseSpace.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
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NextSeq 500 System Guide