2. Click the Next button. The door will open and the source plate gripper stage will extend outside of the Echo Liquid
Handler.
3. Load your source microplate with well A1 closer to the instrument’s inner-left corner. Click the Next button.
The source plate gripper stage retracts into the Echo Liquid Handler and the destination plate gripper stage is extended
outside of the Echo Liquid Handler.
4. Load your destination microplate with well A1 closer to the instrument’s inner-left corner. Click the Next button.
While the destination plate gripper stage retracts the microplate, the source microplate is surveyed. The Echo Liquid
Handler determines the fluid composition and well fluid thickness, and then calculates the fluid volume in each well. For
aqueous solutions, the fluid volume is an approximation and may vary depending on protein and surfactant concentrations,
among other factors.
After the source plate survey is complete, and the destination microplate is positioned above the source microplate, the
liquid transfer process begins.
107 PN | 001-11665
USER GUIDE | Echo® 525 Liquid Handler User Guide Echo Liquid Handler Software