Advanced Liquid Transfer Controls
Each of these controls listed below affect the Echo instrument's behavior while the Echo Liquid Handler client software is being
used to execute fluid transfer protocols. These commands are global in scope, but are set back to default values when the Echo
Liquid Handler client and server are shut down or restarted.
l Survey interval: Select the interval between source plate surveys by choosing a value from the Perform Every Survey drop-
down list.
The default setting of “every 1 plate” causes the source plate to be surveyed every time a new source or destination plate
is loaded. Labcyte recommends that the survey interval remain at 1 for the best transfer precision.
Default: 1
Note: The survey interval can be set to 0; however, with this setting, the protocol will not survey the plate.
Setting the survey interval to 0 turns off plate surveying which is not recommended.
l Assume Homogeneous Plate: When a source plate is surveyed, the Echo 525 Liquid Handler creates a map of the entire
plate. This map is post-processed using a moving average filter if the Assume Homogeneous Plate checkbox is selected.
In a case where a perfectly uniform concentration source plate is being transferred by a source plate calibration that
attempts to measure the fluid concentration for every well, better liquid transfer precision can be obtained using post-
processing. Labcyte recommends that this box remain unchecked for typical operation.
If the Use Predefined checkbox is selected, the Assume Homogeneous Plate feature is unavailable, and its checkbox is
“grayed out.”
Default: Assume homogeneous plate off
l Power Monitoring: Selecting the Power Monitoring checkbox causes the Echo Liquid Handler to monitor the RF power
used to eject each fluid droplet. Labcyte recommends that power monitoring not be enabled by the user unless directed to
do so by Labcyte personnel. Enabling power monitoring adds data processing time to each droplet ejection and decreases
liquid transfer throughput.
Default: Power monitoring off
l Save Plate-Survey Data to Disk: The data collected during a source plate survey is held in cache memory. It is replaced
when the next source plate survey is performed. If the Save Plate-Survey Data to Disk checkbox is selected, the survey
data will be stored on the client PC disk. The data file is stored to <InstallDir>\Labcyte\Medman\Data. This data can
be useful to Labcyte personnel during diagnostic troubleshooting.
Default: Source plate survey data is not saved on the Echo controller
l Save Transfer data to disk: Normally, the data collected during a source plate transfer is deleted when the transfer is
complete. If the Save Transfer data to disk checkbox is selected, the transfer data will be stored on the client PC disk. The
data file is stored to <InstallDir>\Labcyte\Medman\Data. Transfer data can be useful to Labcyte personnel during
diagnostic troubleshooting.
Default: liquid transfer data is not saved to the Echo controller
l Ignore Source Plate Sensor Check: Selecting the Ignore Source Plate Sensor Check checkbox bypasses the source plate
present sensor. This may be useful if the source plate sensor malfunctions so that a transfer protocol can execute without
triggering a “no source plate loaded” error message.
Default: The source plate presence detect sensor is not ignored
l Ignore Destination Plate Sensor Check: Selecting the Ignore Destination Plate Sensor Check checkbox bypasses the
destination plate present sensor. This may be useful when an individual destination plate is not detected by the
destination plate present LEDsensor, or when transferring to a target that does not fit in the destination plate gripper like
the expected SBS compliant targets. When checked, a protocol can execute without triggering a “no destination plate
loaded” error message.
Default: The destination plate presence detect sensor is not ignored
Note: If you choose to select the Ignore Destination Plate Sensor Check checkbox, a plate load error could result
and no error messages will be triggered.
143 PN | 001-11665
USER GUIDE | Echo® 525 Liquid Handler User Guide Echo Liquid Handler Software
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