1. Turn off the coupling fluid pump.
2. In the Diagnostic window of the Echo Liquid Handler software, select the reverse coupling fluid pump direction (Coupling
Pump Direction Reverse). For more information, see Coupling Pump Direction Normal/Reverse.
3. Turn on the coupling fluid pump via the pump power switch. The coupling fluid inside the Echo fluidic circuit will be
pumped back into the coupling fluid bottle.
Note: The fluid returning to the bottle will raise the water level; therefore, watch for potential overflow in the
coupling fluid bottle.
4. Turn off the coupling fluid pump.
5. Return the coupling fluid pump direction to normal. Select Coupling Pump Direction Normal.
6. Unscrew the cap on the coupling fluid bottle and remove the bottle from the bottle caddy.
155 PN | 001-11665
USER GUIDE | Echo® 525 Liquid Handler User Guide Maintenance Procedures