Labcyte Echo 525 - Page 54

384PP Plate
384PP Plus Plate and
384PPTC Plate
384LDVPlus Plate
Echo Qualified
Reservoir
Dead Volume (µL)
1
20 µL 20 µL 4.5 µL 250 µL
Max Fill Volume (µL)
1
65 µL 65 µL
2
14 µL 2800 µL
Wicking No limitation
Max fill of 40 µL for 50-
200% Critical Micelle
Concentration (CMC)
3
Max fill of 8 µL for 2
hours for 50% Critical
Micelle Concentration
(CMC)
3
No limitation
Centrifugation
Recommendation
4
1500 g's for 5
min
1500 g's for 5 min 1500 g's for 5 min 1500 g's for 5 min
Echo Insert Type 2.1 mm 2.1 mm 4.5 mm 2.1 mm
BP 0-2% CMC 0-5% CMC N/A N/A
SP 5-50% CMC 2.5-50% CMC N/A N/A
SPHigh 200% CMC N/A N/A N/A
GP N/A
0-50% Glycerol and 0-5%
CMC
0-50% Glycerol N/A
GPSA N/A
0-50% Glycerol and 5-
14% CMC
N/A N/A
GPSB N/A
0-50% Glycerol and 14-
50% CMC
N/A N/A
BP2 N/A N/A N/A 0-2% CMC
GPSA2 N/A N/A N/A
0-30% Glycerol
and 2-30% CMC
GPSB2 N/A N/A N/A
0-30% Glycerol
and 30-50% CMC
GP
Table 6: Source Microplate Specifications for Echo 525 Liquid Handler
For more details on CMC conversions, see the Critical Micelle Concentrations topic.
1
Varies with fluid properties. May be lower.
2
Max of 40 µL for fluid with >50% Critical Micelle Concentration (CMC) for long term storage, due to wicking.
3
Not recommended for long term storage of fluids containing surfactants, at levels > 50% Critical Micelle Concentration (CMC) .
4
Recommended to remove bubbles caused by the filling process or reagent outgassing.
Note: The 384PP Plus plate is the optimal source plate for the Echo 525 Liquid Handler. The 384PP plate may be used,
but accuracy and precision may be compromised for certain fluid types and destination plates. The 384PP Plus plate and
the Echo Qualified Reservoir are only designed for use with the Echo 525 Liquid Handler and are not compatible with any
other models of Echo Liquid Handlers.
Please contact the Labcyte team at support@labcyte.com, if you have any questions in determining what source plate
options best fit your needs.
Component Description CHAPTER 3 | General Description
54