Note: By design, the 384PP Plus plate has improved drop placement. Therefore, Labcyte recommends using 384PP Plus
plates rather than 384PP plates when transferring to 1536 targets. Use of 1536 well plates as destination plates is
application dependent.
The tables below illustrate the glycerol and CMCpercentage ranges for each plate calibration.
Source Plate Type Destination Plate Type Dispense Type
Dispense Volume Per
Destination Well
Destination Plate Fill
Cycle Time (min)
Reservoir (6RES) 384 1:384 5 µL 9.3
Reservoir (6RES) 1536 1:1536 1 µL 17.2
384PP/384PP Plus 384 384:384 25 nL 2.2
384PP/384PP Plus 384 384:384 1 µL 3.3
384PP/384PP Plus 384 16:384 1 µL 3.7
384PP/384PP Plus 384 48:384 5 µL 10
384LDVPlus 384 384:384 100 nL 3.8
384PP Plus 1536 4x384:1536 25 nL 7.9
Table 7: System Throughput
55 PN | 001-11665
USER GUIDE | Echo® 525 Liquid Handler User Guide Component Description