5.6 Analyzing the Sample (Pre-dilute procedure) (continued)
Pre-dilute procedures are generally less precise than open and closed tube
procedures and results may vary depending on local laboratory procedures and
conditions. Blood cells may shrink and/or swell during the time between mixing
in the beaker and the actual analysis, resulting in compromised values of MCV,
MPV and the distribution between lymphocytes/mid-cells/ granulocytes (with
indirect effect on calculated parameters, e.g. HCT). Thus, the time between
mixing and analysis should be minimized and under no circumstances exceed 60
minutes, since RBC, PLT, HGB and WBC may also be affected.
Externally Pre-
diluted volumes
and preparation
Pre-dilute volumes 4.5ml – 5.0ml. The dilution ratio must always be the same
as the dilution it is calibrated to in order to avoid erroneous results; any dilution
variation in an externally diluted sample will affect the parameter test results.
Prepare pre-dilute sample according to internal documentation and time
limitations section above.
In order to get accurate results always use the same dispenser for calibration and
sample analysis.
This feature is to be used as a precision dispenser for dilution of blood samples.
Dispense amount: 4.5 ml.
Dilution: 20 µl sample in 4.5 ml diluent (1:225)
Follow the instruction below:
Press the [DISPENSE] button from the MENU tab.
Before pressing the pre-dilute start plate make sure that a waste beaker is
placed under the pre-dilute aspiration probe.
Press the pre-dilute start plate (right-side start plate), to enable dispense
mode. (The instrument will fill the waste beaker with a small amount of
diluent, this is to be discarded)
Fill the pre-dilute beaker by pressing the start plate again. If more than one
beaker is to be filled repeat this step.
Prepare pre-dilute sample according to internal documentation and
time limitations section above.
To re-enter analyze mode press [CANCEL] and follow instructions
below to analyze pre-dilute samples.
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