Medonic M16C-US - Startup Sequence

36
Section 5: Sample Analysis
Section Overview
Introduction
This section covers the sample analysis routine, including how to analyze a
sample in the five different modes offered in the Medonic M-Series.
Contents
This section contains the following topics:
Topic
See Page
Preparations before Analysis
36
Startup Sequence
37
Background Count
39
Sample Identification
39
Analyzing the Sample (Open Tube)
40
Analyzing the Sample (Pre-dilution procedure)
42
Analyzing the Sample (Micro Pipette Adapter, MPA)
44
Analyzing the Sample (Cap Piercing Device)
47
Analyzing the Sample (Autoloader)
48
Results
52
5.1 Preparations before Analysis
Sample
collection
 Human venous blood samples should be collected in an EDTA K3 or EDTA
K2 tube in sufficient quantity and be gently mixed immediately after
sampling in order to obtain accurate results. Please follow the
recommendation of the EDTA tube supplier.
 Human capillary blood samples should be collected using only high
precision pipettes recommended by Boule.
Limitations
 Samples drawn in an open tube or vacuum tube should be analyzed within
6 hours for most accurate results.
 Samples drawn into micropipettes should be analyzed within 10 minutes
for most accurate results.
Anticoagulant
recommendation
EDTA K3 (Ethylene Diamine Tetracetic Acid, Tri-potassium) liquid and
EDTA K2 (Ethylene Diamine Tetracetic Acid, Di-potassium) spray-dried
solution. Recommended by ICSH and CLSI (NCCLS).
Handling of
venous blood
samples
 Allowing the blood to equilibrate to the EDTA for 10-15 minutes after
sampling can improve differential results
 The sample should be thoroughly and gently mixed before analysis.
 Improperly mixed samples may cause erroneous results. Mixers are
acceptable for use.
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