Device overview
10
Intended use
The device is a sequencing instrument that measures optical
and electronic signals of the reporting molecules to decode the
sequence information of a piece of DNA or RNA fragment through
the use of instrument specific reagents and flow cells imaging
hardware, and data analysis software. The device is intended for
decoding the sequence of DNAs or RNAs prepared into specific
sequencing libraries such as DNA nanoball libraries. It is not intended
for whole genome sequencing or de novo sequencing.
Contraindication: None.
Working principle
The device adopts the advanced DNA Nano-ball (DNB) and the core
technology of combinatorial probe-anchor synthesis (cPAS) and
uses a regular arrayed flow cell with the special decorated surface.
Each decorated site of the flow cell contains a single DNB, and the
decorated site is evenly arranged on the flow cell, ensuring that the
optical signal of different Nano-ball cannot be interrupted by each
other. Therefore, the accuracy of signal process is improved.
The following figure demonstrates how to make DNBs:
Ad-insert
ed ss DNA
circle
Replicate
DNA Nanoball (DNB)
N×
3×
2×
1×
DNBs
Figure 1 Making DNBs
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