MGI DNBSEQ-G400 - Performance Specifications

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Performance specifications

To comprehensively evaluate the accuracy and reproducibility of the

device for DNA sequencing of different DNBs and DNA sizes, the

national DNA references from different sources of DNBs (human,

bacteria and virus), different genome sizes and reference materials

for GC content are sequenced in three times. The sequencing results

of national references are compared with those of the standard

results. The accuracy rates are all between 99.36% and 99.79% in

the three experiments, and comply with the requirements of the

national DNA reference for performance evaluation on the genetic

sequencer (above 99.0%). Reproducibility of the results from

technical replicates on the device is high for the same reference

DNBs.

Table 3 Test results of genetic sequencer performance

Reference Concentration Repetition Accuracy rate

Escherichia coli DNA 2 μg 1 99.58%

Escherichia coli DNA 2 μg 2 99.56%

Escherichia coli DNA 2 μg 3 99.57%

Escherichia coli DNA 1 μg 1 99.58%

Escherichia coli DNA 1 μg 2 99.58%

Escherichia coli DNA 1 μg 3 99.58%

Escherichia coli DNA 100 ng 1 99.58%

Escherichia coli DNA 100 ng 2 99.57%

Escherichia coli DNA 100 ng 3 99.57%

Escherichia coli DNA precision 0.007%

GC-rich bacteria DNA 2 μg 1 99.72%

GC-rich bacteria DNA 2 μg 2 99.75%

GC-rich bacteria DNA 2 μg 3 99.72%

GC-rich bacteria DNA 1 μg 1 99.73%

GC-rich bacteria DNA 1 μg 2 99.73%

GC-rich bacteria DNA 1 μg 3 99.74%