Structural composition
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The following figure demonstrates how to load DNBs:
DNBs
Flow cell
Decorated sites evenly spread on the flow cell
Each site contains a single DNB
Figure 2 Loading DNBs
The DNB and sequencing reagent are pumped into the sequencing
flow cell through the device’s liquid system. Each DNB combines
with the fluorescence group. The laser excites the fluorescence
group to emit light, and the optical signals are acquired by the
camera. Then the optical signals are processed and transferred to
digital signals, the digital signal is transmitted to and processed by
the computer, so as to acquire the nucleotide sequence of the DNB.
Structural composition
The device consists of the main unit and pre-installed control
software (software version: V1). The main unit includes the main
structure, host, optical system, XYZ-stage, flow cell stage, gas-
liquid system, electric control system, reagent storage system,
power supply system and display system.
The following table describes the function of each component:
Component Description
Main structure Provides the stable support for the main unit.
Host Controls the device, collects, analyzes and stores data.
Optical system Images the fluorescence signal on the flow cell.
XYZ-stage Moves the flow cell and focuses automatically.
Flow cell stage
Connects the flow cell to fluidics lines and controls
the temperature of the flow cell.
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