Performing a wash
47
Wash type Wash solution Frequency
Maintenance
wash
1 M NaCL and 0.05%
Tween-20 for well
No.15, and 0.05%
Tween-20 for other
wells
(First time:
maintenance wash)
z
For SE sequencing: every week
z
For PE sequencing: after each
sequencing run
0.1 M NaOH
(Second time:
maintenance wash)
Laboratory-grade
water
(Third time:
maintenance wash)
Fresh laboratory-
grade water
(Fourth time: regular
wash)
You can use laboratory-grade water such as 18 Megohm (MΩ) water, Milli-Q
water, Super-Q water, or similar molecular biology grade water.
Performing a regular wash
Depending on the frequency of use and sequencing recipe, choose
a wash frequency:
z
If the device has not been used for over 12 hours, perform a
regular wash before sequencing.
z
After each SE sequencing run, perform a regular wash within 12
hours.
Perform the following steps:
1. Before sequencing, log in to your account. Tap Wash in the main
interface.
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