5054747 B 125
Appendix A: Instrument Specifications
This appendix provides specifications for the iD3 basic instrument.
Measurement Specifications
The following tables list the instrument measurement specifications.
96 Wells 384 Wells
Absorbance 0.5 min 1.5 min
Fluorescence Intensity 0.5 min 1.5 min
Luminescence 0.5 min 1.5 min
Table A-1: Read Times using Quick Read (Plate in/out may add 10-15 seconds)
Item Description
Wavelength Range 230 - 1000nm
Wavelength Selection Monochromator tunable in 1nm increments
Wavelength Bandwidth 4.0nm full width half maximum
Wavelength Accuracy ±2.0 nm across wavelength range
Wavelength Repeatability ±1.0nm
Photometric Range 0 - 4.0OD
Photometric Resolution 0.001OD
Photometric Accuracy < ±0.010OD ±1.0%, 0 - 3OD VIS 0 - 3 ODUV
Photometric Precision
(repeatability)
< ±0.003OD ±1.0%, 0 - 3OD VIS0 - 3 ODUV
Stray Light
≤
0.05% at 260nm, 280 nm
Photometric Stabilization Instantaneous
Photometric Drift None (continuous referencing of monochromatic input)
Calibration Automatic before every endpoint read and before the first kinetic
read
Optical Alignment None required
Photodetectors Silicon Photodiode
Table A-2: Absorbance Measurement Specifications of the iD3 instrument
A