SpectraMax iD3 Multi-Mode Microplate Reader User Guide
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Figure 3-8: Injector Maintenance - Prime steps
The Injector Maintenance page enables you to perform the wash and prime steps for the
injectors.
1.
To view the Injector Maintenance page, touch .
The Manual settings enable you control the amount of liquid to move through the
injector tubing for a wash or prime.
2. Lift the instrument's right hood to display the injectors.
3. If reagent is still in the tubing, run a Reverse operation from the Prime settings or the
Manual settings.
4.
Touch to open the microplate drawer.
5. Insert the empty waste plate and the empty strip wells on the microplate drawer. See
Injector Waste Plate and Strip Wells on page 55.
6. Position the injector arm over the waste plate.
Note: The injector arm does not line up with the hole in the waste plate. The
instrument moves the waste plate to the proper position when you touch Wash
or Prime.
7. Use the black pin to move the nozzle from the rear of the injector space to the injector
arm. Lift straight up until the nozzle is free from the instrument.
8. Fill the bottles with enough reagent for your experiment plus at least 2mL to account for
the prime operation and the quick-prime operation before the plate is read, and for the
dead volume in the bottle and the tubing. Place the bottle for injector #1 on the left and
the bottle for injector #2 on the right. See Injector Bottles on page 57.
9. Place the left side snorkel for injector 1 into bottle #1 and the right side snorkel for
injector 2 into bottle #2.
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