Molecular Devices SpectraMax iD3 User Manual

SpectraMax iD3 Multi-Mode Microplate Reader User Guide
90 5054747 B
Spectrum Read Type
Spectrum readings are made using the scanning monochromators of the instrument and can
measure across the spectrum of absorbance wavelengths 230nm to 1000nm. Fluorescent
intensity reads scan excitation wavelengths between 250nm to 830nm and emission
wavelengths between 270nm to 850nm, where the emission wavelength must be a
minimum of 20nm greater than the excitation wavelength. Luminescence reads scan
emission wavelengths between 300nm to 850nm.
Depending on the read mode selected, a spectrum read measures optical density (OD),
%Transmittance (%T), relative fluorescence units (RFU), or relative luminescence units (RLU)
across a spectrum of wavelengths.
Absorbance Read Mode
In the Absorbance (ABS) read mode, the instrument measures the Optical Density (OD) of
the sample solutions.
Absorbance is the quantity of light absorbed by a solution. To measure absorbance
accurately, it is necessary to eliminate light scatter. If there is no turbidity, then
absorbance=optical density.
A=log
10
(I
0
/I)=–log
10
(I/I
0
)
where I
0
is incident light before it enters the sample, I is the intensity of light after it passes
through the sample, and A is the measured absorbance.
For Absorbance reads, you can choose whether to display absorbance data as Optical
Density (OD) or %Transmittance (%T).
Optical Density
Optical density (OD)is the quantity of light passing through a sample to a detector relative to
the total quantity of light available. Optical Density includes absorbance of the sample plus
light scatter from turbidity and background. You can compensate for background using
blanks.
A blank well contains everything used with the sample wells except the chromophore and
sample-specific compounds. Do not use an empty well for a blank.
Some applications are designed for turbid samples, such as algae or other micro-organisms
in suspension. The reported OD values for turbid samples are likely to be different when read
by different instruments.
For optimal results, Molecular Devices recommends that you run replicates for all blanks,
controls, and samples. In this case, the blank value that can be subtracted is the average
value of all blanks.
% Transmittance
%Transmittance is the ratio of transmitted light to the incident light for absorbance reads.
T=I/I
0
%T=100T

Molecular Devices SpectraMax iD3 Specifications

General

Brand	Molecular Devices
Model	SpectraMax iD3
Category	Laboratory Equipment
Language	English