How to merge images in binocular eyepiece of Nikon Eclipse Ti-E Microscope?
If, when viewing through the binocular eyepiece of your Nikon Microscope, the images do not merge into a single image, adjust the interpupillary distance.
How to fix dia illuminator lamp that does not light on Nikon Eclipse Ti-E?
If the dia illuminator lamp does not light on your Nikon Microscope, replace it with a specified lamp.
Why PFS information not displayed on Nikon Eclipse Ti-E Microscope?
If you are not seeing PFS information on the status display panel of your Nikon Microscope, press the DISPLAY switch to display PFS information. Also, attach and connect the PFS Motorized Nosepiece correctly.
Why can't I focus with 20x or 40x objective on Nikon Microscope?
If the image cannot be focused with a 20x or 40x objective on your Nikon Microscope, use a culture vessel with a bottom plate thickness within the objective's correction range.
Why is the image too dark on my Nikon Microscope?
If you're experiencing a vignetted field of view, a completely dark image, or uneven brightness with your Nikon Microscope, make sure all parts are correctly attached. Ensure movable parts like the optical path selector knob, nosepiece, filter sliders, condenser turret, and Bertrand lens in/out lever are in their click-stop positions. Also, the concentric ring on the stage might be interfering with the optical path, so adjust the specimen position. Check that the field diaphragm image is correctly focused on the specimen's focal plane by properly focusing and centering the condenser. The field diaphragm opening should be slightly larger than the field of view. Finally, inspect and clean all optical elements, including the culture vessel, for any dirt or dust.
What to do if Nikon Microscope image cannot be focused?
If the image cannot be focused with your Nikon Microscope, even with the objective at the highest position, ensure that the stage is correctly attached.
Why does my Nikon Eclipse Ti-E Microscope lamp burn out so fast?
If the lamp burns out quickly on your Nikon Microscope, replace it with a specified lamp.
What to do if the lamp does not turn on/off on Nikon Eclipse Ti-E Microscope?
If the lamp on your Nikon Microscope does not turn on or off when you operate the dia illumination ON/OFF switch, replace the lamp with a specified lamp. Also, check the connection of the control cable.
How to fix insufficient brightness in Nikon Microscope's field of view?
If there is insufficient brightness in the field of view of your Nikon Microscope, set the aperture diaphragm to 70 to 80% of the numerical aperture of the objective. Ensure that the field diaphragm image is correctly focused on the specimen's focal plane by properly focusing and centering the condenser. Also, use the optical path selector switches to set the optical path to the observation port.
Why is my Nikon Eclipse Ti-E image yellow-tinged?
If you observe a yellow-tinged image with your Nikon Microscope, move the ND filter into the optical path. You might also need to adjust the brightness control knob to match the lamp rating if the lamp voltage is too low.
General| Type | Inverted Research Microscope |
|---|---|
| Optical System | CFI60 infinity optical system |
| Software | NIS-Elements imaging software |
| Stage | Motorized stage |
| Camera Compatibility | Compatible with various Nikon digital cameras and 3rd party cameras |
| Objectives | Compatible with Nikon CFI60 objectives |
| Focusing | Motorized focus |
| Nosepiece | Motorized 6-position nosepiece |
| Applications | Live-cell imaging, fluorescence |
| Objective Turret | Motorized 6-position |
| Condenser | Motorized condenser |
| Observation Tubes | Binocular and trinocular observation tubes available |
| Camera Port | Multiple camera ports available |
| Dimensions | Varies depending on configuration |
| Weight | Varies depending on configuration |
Illustrates the Ti-E microscope body and its key accessories for identification.
Details the parts and features of the microscope base unit.
Describes the front, left, and right operation panels and their controls.
Identifies and describes the right and rear connector panels.
Details various eyepiece base units, tubes, and their features.
Introduces the PFS Motorized Nosepiece and PFS Offset Controller.
Describes the different types of stages available for attachment.
Details the types of dia pillar illuminators and condensers.
Describes the power supply units and the AC adapter.
Provides an overview of Ti series microscopes and microscopy procedures.
Explains the workflow and steps for bright-field microscopy.
Explains the workflow and steps for phase contrast microscopy.
Explains the workflow and steps for external phase contrast microscopy.
Details the in-focus observation method using the Perfect Focus System (PFS).
Explains procedures for powering the microscope and illumination on and off.
Covers the operation of the dia illumination system, including lamp and brightness.
Describes the functions of the controls on the microscope body's panels.
Explains optical path selection and filter operations.
Details operations for condensers and eyepiece tubes.
Covers focusing mechanism, objective operation, and related controls.
Details operations specific to the pillar illuminator and mechanical stage.
Explains the operation of the Perfect Focus System (PFS).
Lists required tools, installation conditions, and checks for assembly.
Details the steps for installing the microscope body (base).
Explains attachment of eyepiece base units and tubes.
Details attaching the dia pillar illuminator and condensers.
Covers connecting power supply, PFS controller, and AC adapter.
Explains how to register objective information for PFS functionality.
Lists problems related to image quality and viewing, with solutions.
Addresses issues encountered during microscope operation and their fixes.
Lists problems related to power and electrical components with solutions.
Troubleshoots issues specific to the PFS function and its indicators.
Details procedures for cleaning lenses and filters to maintain image quality.
Provides instructions for cleaning the microscope body and disinfection.
Gives guidelines for storing the microscope and recommends periodic inspections.
Lists technical specifications for TI-DH and TI-DS illuminator configurations.
Details specifications for the PFS Motorized Nosepiece and Offset Controller.
Lists specifications for AC adapter, power cords, and safety compliance.
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