Riele 4040+ - Calculation Procedure 12 (Kin;S;Rb)

4040+e_22.docx / 06.10.17
ROBERT RIELE GmbH & Co KG Page 37 6 - CALCULATION PROCEDURES
6.4.12 Calculation procedure 12 (KIN/S/Rb)
Method at which a sample S is measured several times (depending on the number of deltas) in an equidistant
time grid. From the resulting absorbance values an alteration per minute A
S,Minute
is determined by a linear
regression calculation. The reagent blank A
RB,Minute
is measured in the same way as the sample (or entered
directly in U/l) and subtracted from the sample value. This difference is multiplied by a factor F which is determined
by measuring of a standard solution A
ST,Minute
with known concentration C
ST
and under consideration of the
reagent blank A
RB,Minute
.
During the procedure the dialog asks for the use of a reagent blank. The default value is OFF. To continue with-
out reagent blank press [ENTER].
Calculation procedure ..................................................... CP 12
Characteristic ......................................................... KIN / S / Rb
Method .................................................... Kinetic with Standard
Calculation formula ..................... C = F  ( A
S,Minit
- A
RB,Minit
)
Resulting factor ....................... F = C
ST
/ ( A
ST,Minit
- A
RB,Minit
)
Reagent blank ........................................ entering or measuring
Number of deltas ........................................... entering (3 to 19)
Time per delta ........................................ entering (4 s to 255 s)
PHOTOMETER 4040+ # 1001
V2.2a dd/mm/yy D
LAB.: RIELE BERLIN
USER 1: M.MUSTERMANN
DATE: 07/17/17
TIME: 08:44:12
METHOD 32: UREA
PROGRAM: 12
STANDARD: 80.0
WAVELENGTH: 340nm
TEMPERATURE: 37C
DELAY: 3s
DELTAS: 5
TIME/DELTA: 5s
MIN.R^2: 0.998
UNIT: mg/dl
- - - - - - - - - - - -
MEASURE ZERO
Rb[A]: 0.000
Rb/KIN: 0.000
R^2: 0.1973
ST/KIN 1: 0.327
R^2: 0.9996
ST/KIN 2: 0.330
R^2: 0.9989
ST/KIN 3: 0.324
R^2: 0.9994
ST/KIN: 0.327
FACTOR: 244.3
NO. ABS. RESULT
1 0.232 41.5
R^2: 0.9984
2 0.175 81.8
R^2: 0.9997
Start method selection in the main menu.
See chapter:
4.1 - Measurement with programmed methods
4.2 - Measurement with basic methods
In the case of activated printer the print-out of the method data
follows.
The measuring window is shown.
The absorbance value will be constantly refreshed on the display
during the delay time.
At the beginning and at the end of the delay time, the absorbance
values ABS.1 and ABS.2 are measured respectively.
Method procedure:
Insert / measure zero solution
Without reagent blank (insert / measure optionally)
Insert / measure standard 1
Insert / measure standard 2 (optional)
Insert / measure standard 3 (optional)
(Averaged standard)
(Resulting factor)
Insert / measure sample
Numerator / |ABS.1 – ABS.2| / Result
R^2: coefficient of determination, used for linearity control of the
test (see chapter 6.1.7 - Fundamental to the Kinetic…).
Insert / measure sample
A detail print-out of ABS.1, ABS.2 and deltas can be made after
each measurement with [MODE] [MODE] [DETAIL] (see chapter
6.1.7 Fundamental to the Kinetic…).