Operation
43
Checking for aerosol-tight bio-containment
Check the aerosol-tight bio-contain-
ment of your rotor whenever appro-
priate!
Since you cannot yourself carry out
the check with the swinging bucket
rotors, you should monitor seals,
sealing surfaces and screw caps par-
ticularly carefully!
To test, proceed as follows:
• Carefully clean and degrease the rotor chamber
wall, then attach an adhesive white paper strip
(about 4 x 2 cm) so that liquid leaking out of the
rotor may precipitate on it.
• Fill all places of the respective rotor with water
according to the following table. Insert the rotor
into the centrifuge and fasten it.
• Carefully place the amount of test liquid (0.5 %
sodium fluorescein in water) specified in the col-
umn “leakage test” into the lower part of the rotor
within a virtual circle comprising the tube bores
(not the bores themselves) using a pipette or sy-
ringe.
• Place the rotor cover on top and screw it on.
CAUTION: Make sure that there is no spilled test
liquid on the rotor (clean it if necessary)!
• Carry out a test run for 10 minutes at maximum
rotor speed and 23 °C environmental tempera-
ture.
• Check the paper strip under UV light (preferen-
tially in a darkened room):
If there is no detectable fluorescence, aerosol-
tight bio-containment is maintained.
• Before using, rinse the rotor, rotor cover and
cover seal in running water and allow to dry.
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