tecan Infinite 200 PRO User Manual

192 pages

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Instructions for Use for

Infinite 200 PRO

Document Part No.: 30052730

2016-04

Document Revision No.: 1.6

Questions and Answers:

B
Beth RogersAug 14, 2025
What to do if I see error message “CO2 OUT OF RANGE” on my tecan Infinite 200 PRO?
- S
  Stephanie ContrerasAug 14, 2025
  If you receive a “CO2 OUT OF RANGE” error on your Tecan Laboratory Equipment, it could be due to several reasons. One cause is that the gas pressure/flow of CO2 is too high in Manual Mode, meaning the current gas concentration has exceeded 15 % (vol.). The valves will close automatically to stop the gas flow. Reduce CO2 pressure using the pressure reduction valve on the gas cylinder or laboratory gas handling system. Wait until the current gas concentration is below 15 % (vol.) before resuming. Another cause could be that the data cable is not connected to the gas control module and/or Infinite 200 PRO. Ensure the data cable is properly connected.
A
Amber ShermanAug 17, 2025
Why cell application failed on my tecan Infinite 200 PRO?
- J
  Jay CameronAug 17, 2025
  A cell application failure on your Tecan Laboratory Equipment can occur for several reasons. It may be caused by low or no gas availability, in which case you should verify that sufficient gas is available by checking the gauges on the regulator and inspecting for leaks. Another potential cause is a false gas supplied or a false gas control mode selected; ensure you are providing the correct gas and have selected the appropriate gas control mode. Finally, evaporation of cell media can also lead to application failure. To resolve this, apply a suitable gas-permeable adhesive foil, tape, or cover to the microplate.

tecan Infinite 200 PRO Specifications

General

Wavelength Range	230 - 1000 nm
Detection Method	Absorbance, Fluorescence, Luminescence
Software	i-control
Light Source	Xenon flash lamp
Absorbance Wavelength Range	230 - 1000 nm
Fluorescence Excitation Wavelength Range	230 - 850 nm
Fluorescence Emission Wavelength Range	280 - 850 nm
Absorbance Photometric Range	0 - 4 OD
Temperature Control	Ambient +5°C to 65°C
Shaking	Yes
Temperature Range	Ambient +5°C to 65°C
Temperature Accuracy	±0.5°C at 37°C
Plate Formats	6- to 384-well plates

Summary

1. Safety

1.1 Instrument Safety

Essential safety precautions for operating the Infinite 200 PRO to prevent injury or equipment damage.

2. General Description

2.2 Software

2.3 Injectors (Optional)

Information on optional injector modules, their specifications, and setup for liquid dispensing.

3. Installation

3.1 Unpacking and Inspection

Procedures for unpacking the instrument, inspecting contents, and preparing for initial setup.

3.2 Removal of the Transport Locks

Step-by-step guide to safely remove transport locks before operating the instrument.

3.4 Power Requirements

Information on the instrument's power supply voltage range and necessary precautions.

3.5 Switching the Instrument On

Instructions for the initial power-on sequence, including essential precautions before startup.

4. Gas Control Module (Enhanced)

4.1 Safety

Safety precautions specific to the Gas Control Module (GCM), including gas handling and ventilation.

4.2 Gas Control Module Features

4.5 Installing the Gas Control Module

Requirements and procedures for installing the GCM, including environmental, space, and power considerations.

4.6 Operating the Gas Control Module

Instructions on how to operate the GCM, including switching on, selecting modes, and setting gas levels.

4.8 Troubleshooting the Gas Control Module

A table of common problems and errors with the GCM and their corrective measures.

5. Operating the Instrument

5.4 Defining Filter Slides (Infinite F200 PRO only)

Instructions on how to define and manage filter slides and their configurations for measurements.

5.5 Optimizing Fluorescence Measurements

Guidance on optimizing fluorescence measurement results by adjusting instrument parameters and selecting materials.

5.6 FP Measurements

Detailed procedures for performing Fluorescence Polarization (FP) measurements, including calibration.

5.7 Optimizing Absorbance Measurements

Methods for optimizing absorbance measurement results by adjusting parameters like flash settings and settle time.

5.8 Multiple Reads per Well

5.9 Optimizing Luminescence Measurements

5.10 Optimizing AlphaScreen/AlphaLISA Measurements

5.11 Measurements with Injectors

Instructions for priming, washing, and performing measurements using the optional injector system.

5.12 Blanking Measurements

5.13 Cuvette Measurements

7. Quality Control

7.1 Periodic Quality Control Tests

Recommends periodic evaluation of the instrument's performance through specific quality control tests.

7.2 Specifications - Passed/Failed Criteria

Overview of the passed/failed criteria for various specification tests of the Infinite 200 PRO.

7.3 Specifications - Test Instructions

Detailed instructions for performing specification tests for different measurement modes.

7.3.1 Fluorescence Top

7.3.2 Fluorescence Bottom

7.3.3 Time Resolved Fluorescence

7.3.4 Fluorescence Polarization (Infinite F200 PRO only)

7.3.5 Glow Luminescence

7.3.6 Absorbance Accuracy

7.3.7 Absorbance Wavelength Accuracy

7.3.8 Absorbance Baseline Flatness (Infinite M200 PRO)

7.3.9 Absorbance Baseline Flatness (Infinite F200 PRO)

7.3.10 Absorbance Cuvette (Infinite M200 PRO only)

7.3.11 AlphaScreen/AlphaLISA (Infinite F200 PRO only)

8. Cleaning and Maintenance

8.2 Liquid Spills

Procedures for safely handling and cleaning liquid spills on the instrument.

8.3 Injector Cleaning and Maintenance

8.4 Instrument Disinfection

Guidelines for disinfecting instrument parts that may contact infectious material.

8.4.1 Disinfection Solutions

8.4.2 Disinfection Procedure

Step-by-step instructions for disinfecting the instrument and its accessories.

8.4.3 Safety Certificate

8.4.4 Disposal

8.4.6 Disposal of Operating Material

8.4.7 Disposal of the Instrument

Instructions and precautions for disposing of the instrument, including disinfection.

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