tecan Infinite 200 PRO - 7.3.2 Fluorescence Bottom

7. Quality Control
2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 161
7.3.2 Fluorescence Bottom
For the Infinite 200 PRO with the option ‘Fluorescence Bottom’ the following tests
may be performed to prove the specifications:
• Sensitivity (Standard)
• Sensitivity (Enhanced)
• Uniformity
• Precision/Repeatability
These test instructions are valid for the Infinite 200 PRO:
• Infinite F200 PRO
• Infinite M200 PRO
• Standard version
• Spectrally enhanced version
Sensitivity (Standard)
Perform the following measurement to determine the detection limit for
Fluorescein:
Measurement Parameters:
Parameter Setting
Reading Mode Fluorescence Bottom
Ex Wavelength Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Em Wavelength Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
Number of flashes 25
Integration Time 40
Settle Time 0
Gain Optimal
Plate Type GRE96fb
Plate Layout
Pipette 200 µl of 25 nM fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
<> 1 2 3 4 5 6 7 8 9 10 11 12
A
25 nM Fluorescein Blank 25 nM Fluorescein Blank 25 nM Fluorescein Blank 25 nM Fluorescein Blank 25 nM Fluorescein Blank 25 nM Fluorescein Blank
B
C
D
E
F
G
H