tecan Infinite 200 PRO - Measurement with a Simultaneous G-Factor Calibration

5. Operating the Instrument
98 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04
5.6.5 Measurement with a Simultaneous G-Factor Calibration
When Calibrate is selected, the G-factor is determined for the current
measurement parameters and used for the following FP measurement. In order
to perform the G-Factor calibration, please define:
Reference value: select a polarization value for the reference used, e.g. 20 mP
for a 1 nM Fluorescein solution in 0.01 M NaOH.
Reference range: click Change and select the wells filled with the reference.
Blank range: click Change and select the wells filled with the reference blank.
Select Same as measurement blank if the reference blank is the same as the
measurement blank.
Note
By filling in more than one well with polarization references and
reference blanks, the mean values will be calculated and therefore the
calibration result will be more accurate.