5. Operating the Instrument
2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 129
5.14 i-control Examples
Example 1: Dual-Luciferase
®
Assay (Promega Corp.)
For assay details please refer to www.promega.com.
Plate
Select an appropriate plate type. For luminescence measurements,
white microplates are recommended. For this example, a white 96-well
plate was selected.
Part of the plate
(Not shown); can be optionally selected if only part of the plate is to be
processed.
Well
Mandatory for measurements with ‘injection’
Injection (1)
Injector A injects 100 µl with speed 200 µl/sec., refill mode: standard
Wait (Timer)
2 s wait time
Luminescence (1)
Luminescence measurement with 10 s integration time, attenuation
‘none’
Injection (2)
Injector B injects 100 µl with speed 200 µl/sec., refill mode standard
Wait (Timer)
2 s wait time
Luminescence (2)
Luminescence measurement with 10 s integration time, attenuation
‘none’
Move Plate
Plate is moved out after finishing all wells
CAUTION
SWITCH ON THE INSTRUMENT AT LEAST 15 MINUTES BEFORE
STARTING A LUMINESCENCE MEASUREMENT. SOME
COMPONENTS NEED TO WARM UP TO GUARANTEE STABLE
CONDITIONS FOR THE MEASUREMENT.
STOP
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